Journal: Genes & Cancer

Article Title: CD133-positive cancer stem cells from Colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile

doi:

Figure Lengend Snippet: A-B. Cytometry analyses of Nestin, CK20, CD133, Oct3/4, CD44 and CD44v9 on 10% FBS Colo205 growing cells (control) and serum-free growing cultures (week 1 to week 5).

Article Snippet: The purity of sorted cells was evaluated by flow cytometry using FACSCalibur (BD Biosciences) after labeling with anti-human CD133/2 or CD44 antibody (Miltenyi Biotec).

Techniques: Cytometry, Control

Journal: Genes & Cancer

Article Title: CD133-positive cancer stem cells from Colo205 human colon adenocarcinoma cell line show resistance to chemotherapy and display a specific metabolomic profile

doi:

Figure Lengend Snippet: A. Tumorsphere evaluation of CD133+ and CD133- sorted cells. B. Invasiveness of different cell fractions measured using a collagen-based invasion kit (relative DO measured at 560 nm). C. Survival assay after chemotherapy treatment with cisplatin and 5-FU. D. Metabolite quantification after CE-TOF-MS experiments. Only metabolites of interest for Colo205 cells cultured in 10% FBS (control; green bar), CD133+ sorted cells (red bar) and CD44+ sorted cells (yellow bar) are reported here.

Article Snippet: The purity of sorted cells was evaluated by flow cytometry using FACSCalibur (BD Biosciences) after labeling with anti-human CD133/2 or CD44 antibody (Miltenyi Biotec).

Techniques: Clonogenic Cell Survival Assay, Cell Culture, Control

Journal: Nature Communications

Article Title: Mucosal TLR5 activation controls healthspan and longevity

doi: 10.1038/s41467-023-44263-2

Figure Lengend Snippet: Kaplan-Meier survival graphs for female ( a , n = 25 biologically independent animals per group) and male ( b, n = 26 biologically independent animals per group) mice comparing vehicle control mice to those that received intranasally administered to FP fusion protein (6.5 μg) starting at 650 days of age. After the eight times of FP or vehicle administration, the aged mice were analyzed and compared to those of young mice. The results are displayed below: hair condition (c , O-Ctrl: n = 36; O-FP: n = 43 biologically independent animals), bone mineral density of the spine and femur determined by micro-CT ( d , Y: n = 13; O-Ctrl: n = 13; O-FP: n = 14 biologically independent animals), total area of tube formation of bone marrow-derived stem cells ( e , n = 10 biologically independent samples per group), weight of thymus ( f, Y: n = 35 samples; O-Ctrl: n = 36 samples; O-FP: n = 43 samples), number of thymocytes ( g , Y: n = 10 samples; O-Ctrl: n = 7 samples; O-FP: n = 12 samples), phenotypic analysis of naïve T cells (CD3 + CD44 lo CD62L hi ) in splenocytes ( h , Y: n = 10 samples; O-Ctrl: n = 7 samples; O-FP: n = 10 samples), micro-PET images of the mouse brains (left) and quantitative analysis of glucose uptake (right) ( i, Y: n = 12 biologically independent animals; O-Ctrl: n = 12 biologically independent animals; O-FP: n = 13 biologically independent animals), Cognitive functions were assessed with an open field test ( j , Y: n = 16 biologically independent animals; O-Ctrl: n = 8 biologically independent animals; O-FP: n = 16 biologically independent animals), the nest score ( k , Y: n = 9 biologically independent animals; O-Ctrl: n = 9 biologically independent animals; O-FP: n = 8 biologically independent animals), the preference score ( l, Y: n = 10 biologically independent animals; O-Ctrl: n = 7 biologically independent animals; O-FP: n = 8 biologically independent animals), and the passive avoidance test ( m , Y: n = 7 biologically independent animals; O-Ctrl: n = 6 biologically independent animals; O-FP: n = 6 biologically independent animals). Error bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using the Log - rank (Mantel-Cox) test ( a and b ), the unpaired two-tailed t test ( c ), and the one-way ANOVA ( d–m ). ns, not significant; FP, FlaB-PspA fusion proteins; Ctrl, vehicle control group of aged mice; Y, young mice (8 weeks old); SUV, standardized uptake value. Source data are provided as a Source Data file.

Article Snippet: All of the following antibodies for FACS were purchased from BD Biosciences or Novus Biologicals: CD3-FITC, CD44-PE-Cy7, CD62L-APC, TLR5-FITC (Novus Biologicals, Centennial CO, USA).

Techniques: Micro-CT, Derivative Assay, Micro-PET, Two Tailed Test

Journal: Stem Cell Research & Therapy

Article Title: Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment

doi: 10.1186/s13287-022-02968-z

Figure Lengend Snippet: Identification of MSCs derived from human glioma tissues. A and B Adherent growth patterns and fibroblastic morphology of GA-MSCs cultured in MSC media (× 100, scale bars = 1000 µm). C Immunofluorescence showed that GA-MSCs expressed MSC specific markers CD44 and CD105 (× 400, scale bars = 500 µm)

Article Snippet: The cells were incubated with primary antibodies against CD44 and CD105 (1:100, Proteintech, China), then overnight at 4 °C.

Techniques: Derivative Assay, Cell Culture, Immunofluorescence